Skip Navigation



Briefings in Bioinformatics Advance Access published online on March 7, 2006
Briefings in Bioinformatics, doi:10.1093/bib/bbl002
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
7/2/166    most recent
bbl002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Steinhoff, C.
Right arrow Articles by Vingron, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steinhoff, C.
Right arrow Articles by Vingron, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. For Permissions, please email: journals.permissions@oxfordjournals.org

Original Article

Normalization and quantification of differential expression in gene expression microarrays

Christine Steinhoff * and Martin Vingron


  Abstract

Array-based gene expression studies frequently serve to identify genes that are expressed differently under two or more conditions. The actual analysis of the data, however, may be hampered by a number of technical and statistical problems. Possible remedies on the level of computational analysis lie in appropriate preprocessing steps, proper normalization of the data and application of statistical testing procedures in the derivation of differentially expressed genes. This review summarizes methods that are available for these purposes and provides a brief overview of the available software tools.

Keywords: microarray; normalization; low-level analysis; differential gene expression.

Christine Steinhoff is a postdoctoral scientist in the Computational Molecular Biology Department at the Max Planck Institute for Molecular Genetics in Berlin. Her research interest focuses on epigenetic gene regulatory mechanisms especially based on gene expression experimental approaches.

Martin Vingron is the Director at the Max Planck Institute for Molecular Genetics in Berlin and Head of the Department for Computational Molecular Biology. His current research interest lies in utilizing gene expression data as well as evolutionary data for the elucidation of gene regulatory mechanisms.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Brief BioinformHome page
L. L. Elo, J. Hiissa, J. Tuimala, A. Kallio, E. Korpelainen, and T. Aittokallio
Optimized detection of differential expression in global profiling experiments: case studies in clinical transcriptomic and quantitative proteomic datasets
Brief Bioinform, September 1, 2009; 10(5): 547 - 555.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
S. Pounds, C. Cheng, C. Mullighan, S. C. Raimondi, S. Shurtleff, and J. R. Downing
Reference alignment of SNP microarray signals for copy number analysis of tumors
Bioinformatics, February 1, 2009; 25(3): 315 - 321.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
E. Ben-Yaacov and Y. C. Eldar
A fast and flexible method for the segmentation of aCGH data
Bioinformatics, August 15, 2008; 24(16): i139 - i145.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Degenhardt, M. Haubrock, J. Donitz, E. Wingender, and T. Crass
DEEP--A tool for differential expression effector prediction
Nucleic Acids Res., July 13, 2007; 35(suppl_2): W619 - W624.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.